天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (4): 638-642.doi: 10.16333/j.1001-6880.2019.4.013

• 研究简报 • 上一篇    下一篇

UHPLC-QTOF-MSMS比较两种不同基原火炭母的化学成分

吴尤娇1，陈汀波1，姬云青2，程春松1,2，张子凌2，周华1,2，罗培1,2，张志锋1,2*   

  1. 1澳门科技大学中药质量研究国家重点实验室；2澳门科技大学中医药学院，澳门特别行政区 519020
  • 出版日期:2019-04-29 发布日期:2019-04-29
  • 基金资助:

    澳门科学技术发展基金（056/2016/A2）

Chemical comparison of two Polygonum chinense varieties by UHPLC-QTOF-MSMS

WU You-jiao1,CHEN Ting-bo1,JI Yun-qing2,CHENG Chun-song1,2,ZHANG Zi-ling2,ZHOU Hua1,2,LUO Pei1,2,ZHANG Zhi-feng1,2*   

  1. 1State Key Laboratory of Quality Research in Chinese Medicine (Macau University of Science and Technology),Macau Special Administrative Region; 2Faculty of Chinese Medicine,Macau University of Science and Technology,Macau Special Administrative Region,Macau 519020,China
  • Online:2019-04-29 Published:2019-04-29

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摘要： 为评价不同基原火炭母药材的质量差异，本文采用超高效液相色谱-四极杆飞行时间质谱联用（UHPLC-QTOF-MSMS）技术研究火炭母原变种和硬毛火炭母（变种）的化学成分。结果表明：酚酸类、黄酮类成分是火炭母原变种及硬毛火炭母的主要成分；通过与标准品对照，没食子酸、绿原酸、鞣花酸和槲皮苷等4个成分被准确鉴定；其余21个峰通过一级质谱精确质荷比和二级质谱碎片、结合数据库匹配及参考文献等进行结构鉴定。这一分析方法快捷方便，可快速区别火炭母原变种和硬毛火炭母中的化学成分，为全面研究火炭母药材的质量标准和品质评价等提供科学依据。

关键词: 火炭母, UHPLC-QTOF-MSMS, 化学成分

Abstract: In order to evaluate the quality in Polygonum chinense var. chinense and Polygonum chinense var. hispidum from different varieties,the ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MSMS) method was used to evaluate the difference of chemical constituents on Polygonum chinense.The results showed that polyphenols and flavonoids were the main components of both extracts,and four compounds were identified as gallic acid,chlorogenic,ellagic acid and quercitrin based on the reference standards,and 21 compounds were identified or tentatively characterized in P.chinense according to the accurate m/z value analysis of TOF-MS data,and QTOF-MSMS fragment analysis and spectral data from references.This method with the characteristics of fast,sensitivity and efficient,could provide reference for further study in P.chinense,as well as provide scientific basis for the quality standarand research and quality assessment.

Key words: Polygonum chinense, UHPLC-QTOF-MSMS, chemical constituents

中图分类号: 

R917 R284.1

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