天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (增刊1): 121-124.doi: 10.16333/j.1001-6880.2019.S.021

• 开发研究 • 上一篇    下一篇

小分子阿胶纯粉片对小鼠免疫调节功能的影响

卢连华1,周雯1*,张建岭2,郭晓飞2,范轶欧1,张静1,唐慧1,李慧1,陈萌1   

  1. 1山东省疾病预防控制中心,济南 250014;2东阿阿胶股份有限公司 国家胶类中药工程技术研究中心,聊城 252201
  • 出版日期:2019-08-06 发布日期:2019-08-06

Effect of the small molecular Donkey-hide gelatin on immune function of mice

LU Lian-hua1,ZHOU Wen1*,ZHANG Jian-ling2,GUO Xiao-fei2,FAN Yi-ou1,ZHANG Jing1,TANG Hui1,LI Hui1,CHEN Meng1   

  1. 1Shandong Center for Disease Control and Prevention,Jinan 250014,China; 2National Engineering Research Center for Gelatin-based Traditional Chinese Medicine,Dong-E-E-Jiao Co.Ltd.,Liaocheng 252201,China
  • Online:2019-08-06 Published:2019-08-06

摘要: 研究小分子阿胶纯粉片对 ICR 正常小鼠的免疫调节作用。分别以0.18、0.35、1.05 g/kg 小分子阿胶纯粉片经口给予小鼠,连续 30天,通过足趾增厚法、小鼠脾淋巴细胞转化实验检测小鼠细胞免疫调节能力,通过血清溶血素实验、抗体生成细胞实验检测小鼠体液免疫调节能力,通过巨噬细胞吞噬实验、自然杀伤细胞(NK)活力实验测定小鼠非特异性免疫调节能力。结果显示,小分子阿胶纯粉片中剂量组能增加小鼠的抗体生成细胞数(P<0.05);高剂量组能明显促进小鼠淋巴细胞转化率(P<0.01),提高小鼠腹腔巨噬细胞吞噬指数(P<0.05)和NK细胞活性(P<0.05);中、高剂量组能增强小鼠的迟发型变态反应能力( P<0.01) ;低、中、高中剂量组能提高小鼠的血清溶血素水平( P<0.05或 P<0.01)。因此,小分子阿胶纯粉片能明显增强 ICR 小鼠的免疫功能。

关键词: 小分子阿胶纯粉片, 低聚肽, ICR小鼠, 免疫调节

Abstract: To study the effects of the small molecular Donkey-hide gelatin (SDG) on immune function of mice.Methods:Adult female ICR mice were orally administrated with the SDG at doses of 0.18,0.35 and 1.05 g/kg for 30 days consecutively.The cellular immunoregulation of the mice was verified with delay-type hypersensitivity (DTH) test and proliferation and transformation of spleen lymphocyte.The humoral immunoregulation was assessed with antibody-producing cell and serum hemolysin level test.Nonspecific immune regulation was monitored with carbon clearance,macrophage phagocytosis test and activity of natural killer (NK) cells.Spleen index and thymus index of the mice were also determined.The result is that the middle dose group increased the number of antibody producing cells (P<0.05),the high dose group promoted lymphocyte transformation rate (P<0.01) and the macrophage phagocytosis index(P<0.05)and NK cell activity (P<0.05),the delayed type hypersensitivity ability of the middle and high dose groups increased(P<0.01),the level of serum hemolysin were increased in all dose groups (P<0.05 or P<0.01).So the small molecular Donkey-hide gelatin can enhance the immune function of ICR mice.

Key words: small molecular donkey-hide gelatin, oligopeptide, ICR mouse, immune regulation

中图分类号: 

R285.5