天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (9): 1679-1688.doi: 10.16333/j.1001-6880.2025.9.010 cstr: 32307.14.1001-6880.2025.9.010

• 研究简报 • 上一篇    下一篇

地椒草精油体外抗炎和抗氧化活性研究

侯宝龙*，李茂浩   

  1. 陕西中医药大学 陕西中药资源产业化省部共建协同创新中心，咸阳712046
  • 出版日期:2025-09-24 发布日期:2025-09-24
  • 基金资助:
    秦创原中医药产业创新聚集区项目（L2024-QCY-ZYY JJQ-X193）

Anti-inflammatory and antioxidant activities of Thymus quinquecostatus Celak essential oil in vitro

HOU Bao-long*,LI Mao-hao   

  1. Coconstruction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi and Education Ministry,Shaanxi University of Chinese Medicine,Xianyang 712046,China
  • Online:2025-09-24 Published:2025-09-24

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摘要：

探讨地椒草精油（Thymus quinquecostatus Celak essential oil，TQEO）的抗氧化和抗炎活性及其作用机制。通过DPPH、羟基、超氧阴离子自由基清除实验考察了TQEO的体外抗氧化活性；流式细胞术分析TQEO对氧化损伤细胞的凋亡和周期的影响；ELISA检测TQEO对炎症细胞中关键炎症因子表达水平的调控作用；气相色谱-质谱联用技术（gas chromatography-mass spectrometry，GC-MS）对TQEO的化学成分进行分析。在抗氧化活性方面，TQEO对DPPH、羟基和超氧阴离子自由基均表现出显著的清除能力，并能有效降低氧化损伤细胞内的活性氧水平。抗炎活性研究表明，TQEO可浓度依赖性地抑制多种炎症因子的表达，包括肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-1β（interleukin-1β，IL-1β）以及IL-6。通过GC-MS分析鉴定出其主要活性成分，包括百里香酚、香芹酚、4-萜烯醇、丁香酚、松油醇、芳樟醇和薄荷醇等。综上所述，本研究证实了TQEO具有显著的抗氧化和抗炎活性，初步明确了其化学物质组成，为阐明该精油的药效物质基础和作用机制提供了理论依据。

关键词: 地椒草, 精油, 抗氧化活性, 抗炎活性, 化学成分

Abstract:

This study aims to investigate the antioxidant and anti-inflammatory activities of Thymus quinquecostatus Celak essential oil (TQEO) and explore its underlying mechanisms. The antioxidant capacity was evaluated through DPPH, hydroxyl, and superoxide anion radicals scavenging assays. Flow cytometry was employed to analyze the effects of TQEO on apoptosis and cell cycle progression in oxidative stress-induced cells, while ELISA was employed to assess its regulatory effects on key inflammatory cytokines. Chemical composition analysis was performed using gas chromatography-mass spectrometry (GC-MS). The results demonstrated that TQEO exhibited significant scavenging activity on DPPH, hydroxyl, and superoxide anion radicals and effectively reduced intracellular reactive oxygen species  levels in oxidatively damaged cells. Anti-inflammatory evaluation revealed that TQEO dose-dependent inhibited of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6. GC-MS analysis identified its major bioactive constituents, including thymol, carvacrol, terpinen-4-ol, eugenol, terpineol, linalool and menthol. These findings confirm the remarkable antioxidant and anti-inflammatory properties of TQEO and characterize its chemical composition, providing a theoretical foundation for understanding its pharmacodynamic basis and mechanisms.

Key words: Thymus quinquecostatus Celak, essential oil, antioxidation, anti-inflammatory, chemical composition

中图分类号:  R284

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