天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (Suppl1): 70-73.doi: 10.16333/j.10016880.2016.S.018

• 研究简报 • 上一篇    下一篇

柴达木枸杞叶提取物中甜菜碱的鉴定及含量测定

张莉1，党军1，王启兰1，陶燕铎1，刘雪娟2，梅丽娟1*   

  1. 1中国科学院西北高原生物研究所 中国科学院藏药研究重点实验室 青海省藏药研究重点实验室，西宁 810008；内蒙古自治区阿拉善盟林业治沙研究所，阿拉善左旗 750300
  • 出版日期:2016-06-30 发布日期:2017-03-02

Identification and Determination of Betaine in Extract of Lycium barbarum L.Leaf from Qaidam Basin

ZHANG Li1,DANG Jun1,WANG Qilan1,TAO Yanduo1,LIU Xuejuan2,MEI Lijuan1*   

  1. 1 Northwest Institute of Plateau Biology,Chinese Academy of Sciences / Key Laboratory of Tibetan  Medicine Research,Chinese Academy of Sciences / Qinghai Key Laboratory of Tibetan Medicine Research, Qinghai Xining 810008,China;2 Alxa Forestry and Desert Control Research Institute,Inner Mongolia Alxa Left Banner 750300,China
  • Online:2016-06-30 Published:2017-03-02

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摘要： 对柴达木枸杞（Lycium barbarum L.）叶提取物中甜菜碱进行鉴定及含量测定，着重建立柴达木枸杞叶提取物中甜菜碱的含量测定方法。采用HPLC法建立枸杞叶提取物中甜菜碱的含量测定方法。HPLC色谱分析条件：色谱柱：Hypersil NH2柱（250×4.6 mm，5 μm）；流动相：0.1%甲酸乙腈：0.1%甲酸水（85∶〖KG-*2〗15）；流速：0.9 mL/min；柱温：30 ℃；检测波长：195 nm。HPLC法测定结果表明：甜菜碱在0.5~4.5 μg范围内线性关系良好，平均回收率为101.14%，RSD为0.30%。柴达木枸杞叶提取物中甜菜碱的含量不得低于10%。该方法可以准确地进行定性、定量检测，操作简便快速、结果可靠、重现性好，可完善在柴达木枸杞叶化学成分方面的研究，并有效控制其质量。

关键词: 枸杞叶, 高效液相色谱, 甜菜碱, 含量测定

Abstract: In this study,betaine in the extract of Lycium barbarum L.leaf from Qaidam Basin was identified and quantified by high performance liquid chromatography (HPLC).The chromatographic analysis conditions were as follows:using Hypersil NH2 (250×4.6 mm,5 μm) column with 0.1% formic acid in acetonitrile (85%) and 0.1% formic acid in water (15%) as mobile phases.The flow rate was set at 0.9 mL/min,and column temperature was maintained at 30 ℃,UV detection wavelength was 195 nm.Betaine showed good linearity within the range of 0.54.5 μg.The average recovery was 101.14% and the relative standard deviation (RSD) was 0.30%.The content of betaine in the extract of L.barbarum leaf from Qaidam Basin was no less than 10%.The developed HPLC method was simple,rapid,reliable as well as reproducible.It can be used for the qualitative and quantitative determination of betaine in L.barbarum leaf from the Qaidam Basin and hence to control its quality effectively.

Key words: Lycium barbarum L. leaf, high performanceliquid chromatography, betaine, content determination

中图分类号: 

Q946.88

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