HPLC-ELSD法测定家桑野桑植株中1-脱氧野尻霉素的含量

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (3): 490-493.

HPLC-ELSD法测定家桑野桑植株中1-脱氧野尻霉素的含量

宋婕，邓洁丽，周光雄*

暨南大学药学院中药及天然药物研究所广东省高校中药药效物质基础与创新药物研究重点实验室，广州 510632

收稿日期:2011-09-27 出版日期:2011-06-25 发布日期:2011-08-08
通讯作者: guangxzh@sina.com
基金资助:
广东省教育部产学研结合项目（2009B090600117）

Determine the Content of 1-Deoxynojirimycin in Mulberry Resources by HPLC-ELSD

SONG Jie, DENG Jie-li, ZHOU Guang-xiong*

Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research,Institute of Traditional Chinese Medicine and Natural Products,College of Pharmacy,Jinan University,Guangzhou 510632,China

Received:2011-09-27 Online:2011-06-25 Published:2011-08-08

摘要/Abstract

摘要：

采用HPLC-ELSD法对桑植株各部位所含的1-脱氧野尻霉素（DNJ）进行了含量测定，使用TSKgel Amide-80（4.6 mm×250 mm，5 μm）色谱柱，流动相为：乙腈-水（84:16，6.5 mM/L醋酸铵），流速为1.00 mL/min，柱温：25 °C，线性范围为0.5 μg~20 μg（r = 0.9991，n = 6），平均回收率为94.55%（n = 5），RSD=1.99。测定结果表明，野桑叶、嫩枝和根皮部位的DNJ含量较高，而家桑的叶、枝皮和根皮中含量较高。本方法准确度高、重现性好，能快速检测桑树资源及类似植物和相关产品中1-脱氧野尻霉素的含量。

关键词: 1-脱氧野尻霉素, 高效液相色谱法, 蒸发光散射检测器, 桑

Abstract:

The content of 1-deoxynojirimycin (DNJ) in mulberry resources was determined by HPLC with an evaporative light scattering detector (ELSD). The HPLC method was established using TSKgel Amide-80 (4.6 mm×250 mm, 5 μm) column with acetonitrile–water (84:16) as mobile phase and the water containing 6.5 mM/L ammonium acetate. The flow rate was 1.00 mL/min, column temperature 25 °C. The standard curve for DNJ using ELSD was linear in the range of 0.5 μg ~ 20 μg, r = 0.9991 (n = 6). The average recovery was 94.55% (n = 5), RSD=1.99%. The results showed that the contents of DNJ in mulberry leaves, root barks, and twigs were higher than other parts of Morus australis. And the contents of DNJ in mulberry leaves, branch phloem, and root barks were higher than other parts of M. alba. The method was accurate and reproducible. It could be used for the rapid determination of DNJ in mulberry resources and other related products.

Key words: 1-deoxynojirimycin, HPLC, ELSD, mulberry

中图分类号:

Q946.91 R284.1

宋婕，邓洁丽，周光雄. HPLC-ELSD法测定家桑野桑植株中1-脱氧野尻霉素的含量[J]. 天然产物研究与开发, 2011, 23(3): 490-493.

SONG Jie, DENG Jie-li, ZHOU Guang-xiong. Determine the Content of 1-Deoxynojirimycin in Mulberry Resources by HPLC-ELSD[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(3): 490-493.

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