天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl1): 96-99.

• 开发研究 • 上一篇    下一篇

青翘精油的抑菌及抗氧化性能研究

石海燕,曹志钦,周娅静,王青,马雪梅*   

  1. 中北大学化工与环境学院化学工程系,太原 030051
  • 出版日期:2014-12-30 发布日期:2015-03-04

Study on the Bacteriostasis and Antioxidant Activities of Green Fructus Forsythiae Essential Oil

SHI Hai-yan, CAO Zhi-qing, ZHOU Ya-jing, WANG Qing, MA Xue-mei*   

  1. Department of chemical engineering,School of Chemical Engineering and Environment,North University of China,Taiyuan 030051,China
  • Online:2014-12-30 Published:2015-03-04

摘要: 采用超临界CO2流体萃取技术提取青翘精油,通过体外试验研究青翘精油对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的最低抑菌浓度(MIC)以及研究温度、pH对精油抑菌效果的影响。同时采用羟基自由基清除、DPPH自由基清除、磷钼络合物法以及硫氰酸铁法四种体外抗氧化方法,以合成抗氧化剂BHT和天然抗氧化剂VC作对照,评价青翘精油的抗氧化活性。实验结果表明,青翘精油的抑菌效果随pH的减小而增大,随温度的升高而增强。同时,青翘精油在不同的抗氧化评价方法中均体现出一定的抗氧化活性,但总体抗氧化能力要弱于BHT。

关键词: 青翘精油, 抑菌, 抗氧化

Abstract: Green Fructus forsythiae essential oil was extracted by supercritical carbon dioxide extraction(SCDE)technique.The minimum inhibitory concentration(MIC)of the essential oil were determined by the test in vitro on the tested strains(Escherichia coli,Staphylococcus Aureus and Bacillus Subtilis).The effects of temperature and pH on bacteriostasis of the essential oil were also studied.Hydroxyl radical scavenging method,DPPH radical scavenging method,phosphomolybdenum complex assay and ferric thiocyanate method were used to evaluate antioxidant activities of Green Fructus forsythiae essential oil and the results were compared with those of antioxidants such as butylated hydroxytoluene(BHT)and ascorbic acid(VC).The experimental results showed that the antibacterial effect of Green Fructus forsythiae essential oil enhanced with pH decreasing or with the increase of temperature.Results also showed that Green Fructus forsythiae essential oil in different antioxidant evaluation methods were reflected with certain antioxidant activities,but the overall antioxidant capacity was weaker than BHT.

Key words: Green Fructus forsythiae essential oil, bacteriostasis, antioxidation

中图分类号: 

R284.1 Q946.8