关键词: 黑小豆种皮, 花色苷, 提取, 抗氧化活性

Abstract: In order to explore new anthocyanins resources,extraction process for anthocyanin pigments from Vigna angularis (Willd.) Ohwi et Ohashi hulls was developed and optimized by single factor and L₉(3⁴) orthogonal experiments.The optimal conditions for the extraction of anthocyanins from V.angularis hulls was 60% ethanol as extraction solvent,solid-liquid ratio of 1:20 (g:mL),temperature of 40 ℃ and pH 2.0.Under these extraction conditions,the highest extraction amount for anthocyanins was 5.912 mg/g with extraction yield of 19.1% and purity of 3.06%.V.angularis hulls crude extract showed a strong O₂^·-, ·OH and DPPH radical scavenging ability. V. hulls can be utilized as a new type of anthocyanins resource.

Key words: Vigna angularis (Willd.) Ohwi et Ohash hulls, anthocyanins, extraction, antioxidant activity