天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (11): 2028-2034.doi: 10.16333/j.1001-6880.2025.11.006 cstr: 32307.14.1001-6880.2025.11.006

• 研究论文 • 上一篇    下一篇

垫状卷柏中一个新的环肽

陈小明1,2,3，贺  茜1，吕敬崑3，谢  夷1，姜红宇1,2*   

  1. 1湖南科技学院化学与生物工程学院；2湖南省银杏工程技术研究中心；3湖南恒伟药业股份有限公司，永州 425100
  • 出版日期:2025-11-27 发布日期:2025-11-26
  • 基金资助:
    湖南省自然科学基金(2024JJ7198)

A new cyclopeptide from Selaginella pulvinata

CHEN Xiao-ming1,2,3,HE Xi1,LYU Jing-kun3,XIE Yi1,JIANG Hong-yu1,2*   

  1. 1School of Chemistry and Bioengineering,Hunan University of Science and Engineering；2Hunan Provincial Engineering Research Center for Ginkgo Folium,Hunan University of Science and Engineering;3Hunan HENGWEI Pharmaceutical Co.,Ltd.,Yongzhou 425100,China
  • Online:2025-11-27 Published:2025-11-26

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摘要：

研究垫状卷柏全草（Selaginella pulvinata）的化学成分及其抗肿瘤活性，采用硅胶、MCI、Sephadex LH-20柱色谱和半制备HPLC等多种分离技术对垫状卷柏95%乙醇提取物进行分离纯化，并根据核磁共振波谱、高分辨质谱等方法鉴定化合物结构。结果从中分离并鉴定了6个化合物，分别为pulvpeptin B（1）、selaginellin G（2）、selaginellin P（3）、selagibenzophenone A（4）、N-(2E)-3-(3,4-dihydrophenyl)prop-N1′-(4-aminobutyl)-3- pyrrole-formaldehyde（5）、环-(L-羟脯氨酸-L-亮氨酸)二肽（6），其中化合物1为新的环八肽，化合物5和6为首次从垫状卷柏中分离得到。通过MTT法评价了化合物1~6的抗肿瘤活性，结果表明化合物1~5对人肝癌细胞（HepG2）和人肺癌细胞（A549）具有不同程度的增殖抑制作用。其中，化合物1对HepG2和A549均显示出较强的抑制活性，其IC50值分别为10.24 ± 0.33 、9.32 ± 0.81 μmol/L。

关键词: 垫状卷柏, 化学成分, 环肽, 抗肿瘤活性

Abstract:

To study the chemical constituents of the whole herb of Selaginella pulvinata and their antitumor activity. Six compounds were isolated from the 95% ethanol extract of S. pulvinata by silica gel, MCI, Sephadex LH-20 column chromatography, as well as semi-preparative HPLC methods. Their structures were identified by NMR and HR-ESI-MS methods as pulvpeptin B (1), selaginellin G (2), selaginellin P (3), selagibenzophenone A (4), N-(2E)-3- (3,4-dihydrophenyl)prop-N1′-(4-aminobutyl)-3-pyrrole-formaldehyde (5), and cyclo-(L-Hyp-L-Leu) (6). Among them, compound 1 was a new cyclopeptide, and compounds 5 and 6 were obtained from S. pulvinata for the first time. The antitumor activities of compounds 1-6 was determined by MTT assay. The results showed that compounds 1-5 had various inhibitory effects on the proliferation of human liver cancer cells (HepG2) and human lung cancer cells (A549). Compound 1 exhibited the significant inhibitory activity against HepG2 cells and A549 cells with IC50 values of (10.24 ± 0.33), (9.32 ± 0.81) μmol/L, respectively.

Key words: Selaginella pulvinata, chemical constituent, cyclopeptide, antitumor activity

中图分类号:  R284.1

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