天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (1): 110-115.doi: 10.16333/j.1001-6880.2019.1.017

• 开发研究 • 上一篇    下一篇

金银花配方颗粒、标准汤剂含量测定及指纹图谱研究

朱敏1，魏惠珍1,2，金浩鑫2，吴柳瑾2，鄢星1，林苗苗1，张晓男1，饶毅1,2*   

  1. 1江西中医药大学，南昌 330004；2中药固体制剂制造技术国家工程研究中心，南昌 330006
  • 出版日期:2019-01-28 发布日期:2019-01-28
  • 基金资助:

    江西省重点研究计划（2017ACG70010）

Content determination and fingerprint of Lonicera japonica formula granules and standard decoctions

ZHU Min1,WEI Hui-zhen1,2,JIN Hao-xin2,WU Liu-jin2,YAN Xing1,LIN Miao-miao1,ZHANG Xiao-nan1,RAO Yi1,2*   

  1. 1Jiangxi University of Traditional Chinese Medicine,Nanchang 330004; 2The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine,Nanchang 330006
  • Online:2019-01-28 Published:2019-01-28

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摘要： 本文主要为金银花配方颗粒的质量评价提供依据，并建立金银花配方颗粒、标准汤剂含量测定及HPLC指纹图谱的研究方法。主要采用HPLC法测定金银花饮片、标准汤剂、配方颗粒绿原酸、木犀草苷含量，建立三者指纹图谱共有模式。结果显示金银花饮片、标准汤剂、配方颗粒中绿原酸、木犀草苷含量差异较大；分别建立金银花饮片、标准汤剂、配方颗粒的指纹图谱；金银花配方颗粒HPLC指纹图谱中的12个共有峰均可在标准汤剂中追踪到，有11个可以在饮片中得到追踪，并指认出绿原酸、芦丁、木犀草苷3种成分。表明所建立的定性、定量分析方法简便，准确度高，专属性强，对金银花配方颗粒的鉴定及质量控制具有指导意义和参考价值。

关键词: 金银花饮片, 标准汤剂, 配方颗粒, 含量测定, 指纹图谱

Abstract: This paper established the content determination of Lonicera japonica formula granules,standard decoction and HPLC fingerprint study method,which provided the basis for the quality evaluation of Lonicera japonica formula granules.HPLC method was used to determine the content of Lonicera japonica herb,standard decoction,formula granules chlorogenic acid and luteoloside,The common pattern of the three fingerprints was established.The results showed that the contents of chlorogenic acid and luteoloside in Lonicera japonica herb,standard decoction and formula granules were significantly different.Fingerprint of Lonicera japonica herb,standard decoction and formula granules were established respectively.A total of 12 peaks in HPLC fingerprint of Lonicera japonica formula granules can be traced in standard decoction,11 were tracked in the herb and to identify the chlorogenic acid,rutin and luteoloside of 3 components.The indicates that the method of qualitative and quantitative analysis is simple,accurate,and specific,which has guiding significance and reference value for the identification and quality control of formula granules.

Key words: Lonicera japonica herb, standard decoction, formula granules, content determination, fingerprint

中图分类号: 

R284.1

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