天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (8): 1372-1377.doi: 10.16333/j.1001-6880.2019.8.010

• 研究简报 • 上一篇    下一篇

不同产地罗汉果总皂苷、甜苷V和硒含量及指纹图谱研究

杨凤轩1，李刚2,3，袁汉文1*，夏祥华2，夏勉3*，周琼4   

  1. 1广西民族大学 广西高校微生物与植物资源利用重点实验室，南宁 530006；2广西药用植物园，南宁 530023；3苍溪县国家现代农业示范区院士工作站，苍溪 628400；4广西大学农学院，南宁 530004
  • 出版日期:2019-09-10 发布日期:2019-09-10
  • 基金资助:

    四川省科技计划项目重点研发（2017NZ0073）；国家自然科学基金（31260359；31460689）；广西民族大学研究生教育创新计划（gxun-chxps201769）

Studies on the contents of total saponins,glycoside V and selenium in Siraitia grosvenorii from different origins and their fingerprints

YANG Feng-xuan 1,LI Gang 2,3,YUAN Han-wen1*, XIA Xiang-hua2,XIA Mian3*,ZHOU Qiong4   

  1. 1Guangxi University for Nationalities,Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources,Nanning 530006,China; 2Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China; 3Cangxi Xingke Modern Agricultural Science and Technology Research Institute Co.,Ltd.,Cangxi 628400,China; 4College of Agricultural,Guangxi University,Nanning 530004,China
  • Online:2019-09-10 Published:2019-09-10

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摘要： 对不同产地同一品种收获期罗汉果总皂苷、甜苷V和硒的含量进行比较，了解罗汉果的品质特点与产地的相关性，科学指导种植区域的生产规划，明确不同区域罗汉果的品质特点和开发等直接关系到罗汉果深加工产业的质量与发展。采用香草醛-浓硫酸试剂分光光度比色法分别测量各罗汉果果实样品中总皂苷的含量，采用高效液相色谱法测量甜苷V的含量，采用氢化物原子荧光光谱法测量其中硒的含量，并对各指标进行方差分析和差异显著性的多重比较。结果表明，各样品总皂苷含量在2.42%~3.34%之间，甜苷V含量在0.68%~1.41%之间，硒含量在5.634~41.462 μg/kg之间；来源于不同产地的罗汉果样品，其总皂苷含量差异并不显著，但甜苷V和硒含量分别表现出显著差异；广西、湖南和四川，罗汉果果实总皂苷和甜苷V含量均可达到药典指标要求。因此，罗汉果具有较广的种植区域，但不同产地甜苷V含量差异相对显著，暗示果实甜苷V的转化效率或成熟度具有一定差异，宜根据产地及年度气候条件适当调整收获期，以满足不同加工利用的需要。

关键词: 罗汉果, 甜苷V, 总皂苷, 硒, 指纹图谱

Abstract: The contents of total saponins,mogroside V and selenium in fruits were separately compared with the same variety of Siraitia grosvenorii with the same harvesting period from different areas.Understanding the correlation between quality characteristics and origin of Siraitia grosvenorii,guiding the production planning of planting areas scientifically,and clarifying the quality characteristics and development of Siraitia grosvenorii in different areas are directly related to the quality and development of Siraitia grosvenorii intensive processing industry.The total saponin content of each Siraitia grosvenorii fruit sample was measured by vanillin-concentrated sulfuric acid reagent combined with spectrophotometric colorimetric method.The content of mogroside V was measured by high performance liquid chromatography,and the content of selenium was measured by hydride atomic fluorescence spectrometry.Then,analysis of variance and multiple comparisons with Duncan test for each parameter were conducted.It showed that,the content of total saponins,glucoside V and selenium were 2.42% to 3.34%,0.68% to 1.41%,5.634-41.462 μg/kg,respectively;there was no significant difference in the total saponins content between the samples from different origins,but there were significant differences in the content of sweet glucoside V and selenium;the content of total saponins,mogroside V,total saponins and selenium in Siraitia grosvenorii from Guangxi and Hunan to Sichuan met the requirements of pharmacopoeia of P.R.China.Therefore,Siraitia grosvenorii has wider planting areas,yet the content of mogroside V from different areas was different,significantly,which implied that the conversion efficiency of the fruit mogroside V or maturity of the fruitswere different,and the harvest period should be appropriately adjusted according to the planting region and the corresponding annual climatic conditions to meet the needs of different processing and utilization.

Key words: Siraitia grosvenorii, mogroside V, total saponins, selenium, fingerprints

中图分类号: 

R284 Q946

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