银杏外种皮与垂序商陆叶提取物及二者复配剂对小菜蛾杀虫活性研究

doi:10.16333/j.1001-6880.2020.10.016

天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (10): 1747-1753.doi: 10.16333/j.1001-6880.2020.10.016

银杏外种皮与垂序商陆叶提取物及二者复配剂对小菜蛾杀虫活性研究

于凌一丹#，向阳春#，蹇宁彬，何钦，汤瑶，廖阳*，闫荣玲*

1湖南科技学院化学与生物工程学院；2湘南优势植物资源综合利用湖南省重点实验室；3湖南省银杏工程技术中心，永州 425199

出版日期:2020-10-28 发布日期:2020-10-30
基金资助:
湖南省青年骨干教师资助项目（湘教通［2018］574号）；湖南省自然科学基金（湘基金委［2020］1号2020JJ4325）；永州市科技计划（永科发［2019］15号）；湖南科技学院青苗支持计划（湘科院校发［2019］47号）

Insecticidal activity of extracts of Ginkgo biloba sarcotesta and Phytolacca americana leaf and their mixture against Plutella xylostella

YU Lin-yi-dan#,XIANG Yang-chun#,JIAN Ning-bin,HE Qin,TANG Yao,LIAO Yang*,YAN Rong-ling*

1College of Life Sciences and Chemistry Engineering,Hunan University of Science and Engineering;2Key Laboratory of Comprehensive Utilization of Dominant Plant Resources in Southern Hunan;3Hunan Provincial Engineering Research Center for Ginkgo biloba, Yongzhou 425199,China

Online:2020-10-28 Published:2020-10-30

摘要/Abstract

摘要：

为更好开发利用垂序商陆与银杏两种植物资源，本研究对银杏外种皮（Ginkgo biloba sarcotesta，Gbs）及垂序商陆叶（Phytolacca americana leaf，Pal）提取物的单方及复配剂对小菜蛾的杀虫活性进行了测试和比较。结果发现，银杏外种皮与垂序商陆叶提取物均能对小菜蛾表现出良好灭杀活性，且灭杀活性随处理时长及提取物浓度的增加而增加，各浓度下银杏外种皮提取物的小菜蛾杀虫活性均优于垂序商陆叶提取物，8 mg/mL浓度下处理72 h后二者的小菜蛾校正死亡率分别为74.7%（Gbs）与64.8%（Pal）；二者毒力回归方程分别为y=1.584x-1.243（Gbs）与y=1.337x-0.629（Pal），LC₅₀分别为2.95 mg/mL（Gbs）与6.09 mg/mL（Pal）；复配剂的小菜蛾灭杀活性随二者配比变化而变化，最佳配比为1∶11（Pal∶Gbs），LC₅₀为1.24 mg/mL（低于二者各自单方的LC₅₀，毒力指数为491.13 mg/mL（明显高于二者单剂毒力指数之和），共毒系数为152.92（表明复配增效作用明显）。研究结果可为利用垂序商陆叶与银杏外种皮协同开发新型生物农药提供实验依据。

关键词: 生物农药, 半致死浓度, 杀虫活性, 共毒系数, 毒力指数, 银杏, 垂序商陆

Abstract:

In order to have a better exploitation and utilization for Phytolacca americana and Ginkgo biloba sarcotesta,the insecticidal activity of the extract of Phytolacca americana leaf (Pal) and Ginkgo biloba sarcotesta (Gbs) and their mixture against Plutella xylostella were tested and compared in the present paper.Results showed that,both of the two kinds plant extracts showed good insecticidal activities against Plutella xylostella,and the activity raised with the increasing of treating time and extract concentration.The extract of Gbs performed better than that of Pal at all concentrations,and the corrected mortality were 74.7% (Gbs) and 64.8% (Pal) after 72 hours treatment with 8 mg/mL concentration.The virulence regression equation were y=1.584x-1.243(Gbs) and y=1.337x-0.629(Pal) while the LC₅₀ were 2.95 mg/mL(Gbs) and 6.09 mg/mL(Pal).The insecticidal activity of the two extracts’ mixture varied with the mixing ratio and the optimum ratio was 1∶11 (Pal∶Gbs).The mixture’s LC₅₀ was 1.34 mg/mL which was lower than that of both single extract while the virulence index was 491.13 mg/mL which was higher than the sum of two single extracts’ virulence index.The co-toxicity coefficient value of 152.92 suggested a significant synergistic effect existed in mixing treatment.These results could provide some references for the new bio-pesticides exploitation based on the extract of Pal and Gbs.

Key words: biopesticides, LC₅₀, insecticidal activity, co-toxicity coefficient, toxicity index, Ginkgo biloba, Phytolacca americana

中图分类号: Q945.1

于凌一丹#, 向阳春#, 蹇宁彬, 何钦, 汤瑶, 廖阳, 闫荣玲. 银杏外种皮与垂序商陆叶提取物及二者复配剂对小菜蛾杀虫活性研究[J]. 天然产物研究与开发, 2020, 32(10): 1747-1753.

YU Lin-yi-dan#, XIANG Yang-chun#, JIAN Ning-bin, HE Qin, TANG Yao, LIAO Yang, YAN Rong-ling. Insecticidal activity of extracts of Ginkgo biloba sarcotesta and Phytolacca americana leaf and their mixture against Plutella xylostella[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2020, 32(10): 1747-1753.

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银杏外种皮与垂序商陆叶提取物及二者复配剂对小菜蛾杀虫活性研究

Insecticidal activity of extracts of Ginkgo biloba sarcotesta and Phytolacca americana leaf and their mixture against Plutella xylostella

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