NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2017, Vol. 29 ›› Issue (增刊1): 77-81. doi: 10.16333/j.1001-6880.2017.S.016

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Determination of Radix Radix Total Sugar Content by Titration

ZHANG Lu-Xin1,LI De-kun2,3,ZHOU Da-zheng2,3,YANG Yu-wu2,3,YU Bo-yang1*,JU Ai-chun2,3*   

  1. 1China Pharmaceutical University,Nanjing 211198,China;2Tianjin Key Laboratory of Safety Evaluation Enterprise of TCM Injections;3Tianjin Tasly Pride Pharmaceutical Co.,Ltd.,Tianjin 300402,China
  • Online:2017-08-02 Published:2017-08-02

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Abstract: The aim of this study is using sodium periodate titration method to establish a determination methods of total sugar content in Radix Ophiopogonis.Medicinal powder extracted by purified water and overSPE product column,under the condition of acid,water bath and after reaction with NaIO4 solution,so that the consumption of sodium thiosulfate solution is reduced，thus calculated total sugar content.The results show 6 batches of Radix Ophiopogonis total sugar content were 623.64,529.86,548.61,633.01,482.97,557.99 mg/g.After study methodology,the determination method is rapid,accurate and can be used to determine Radix Ophiopogonis total sugar content.

Key words: titration, Radix Ophiopogonis, total sugar content