NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (12): 2039-2048.doi: 10.16333/j.1001-6880.2018.12.002

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Extraction of Proanthocyanidins from Cynomorium songaricum Rupr.and Its Antioxidant and Antiglycation Activities

ZHANG Xi-feng1,2*,CHEN Yu-di1,CUI Jing1,CHENG Wen-hui1   

  1. 1College of Agriculture and Biotechnology,Hexi University;2Key Laboratory of Hexi Corridor Resources Utilization of Gansu Zhangye,Zhangye 734000, China
  • Online:2019-01-02 Published:2019-01-02

Abstract: Proanthocyanidins obtained by different extraction methods were researched on the yield,antioxidant and antiglycation activities.Proanthocyanidins from Cynomorium songaricum Rupr.was extracted by ultrasound-assisted extraction (UAE),ethanol-ammonium sulfate two-phase extraction,water extraction,microwave-assisted extraction (MAE),and enzymatic hydrolysis,respectively.The content was determined by Vanillin-HCl method.DPPH· scavenge activity and hydroxyl radical assay were conducted to evaluate the antioxidant abilities of proanthocyanidins prepared by different methods.Bovine serum albumin (BSA)-Glucose and BSA-methyglyoxal (MGO) models were applied to the measurement of antiglycation capacity.The results indicated that MAE resulted in higher yields,15.00% and a shorter extraction time of 19 min.In terms of DPPH radical scavenging activities,MAE showed the best antioxidant capacity,followed by enzymatic hydrolysis,UAE,ethanol-ammonium sulfate two-phase extraction,water extraction.MAE showed the best the scavenging rate of OH,followed by enzymatic hydrolysis,UAE,water extraction,and ethanol-ammonium sulfate two-phase extraction.The proanthocyanidins prepared by MAE method had excellent antiglycation activity in BSA-glucose and BSA-MGO model,respectively.Antioxidant showed no correlation with antiglycation activity.

Key words: Cynomorium songaricum Rupr., proanthocyanidins, microwave-assisted extraction, antiglycation

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