Abstract:

To investigate the content of total flavonoids and the monthly change trend in Folium Hibiscus.The antioxidant activity of total flavonoids in Folium Hibiscus was determined by total antioxidant test,DPPH method,ABTS method and PTIO method,using Vc as control.The change trends of content of four flavonoids in Folium Hibiscus were also studied by UFLC,and the correlation between the four flavonoids and antioxidant activity was analyzed.The results showed the monthly change trends of antioxidant activity of flavonoids in Folium Hibiscus were different among the four methods,but there was a positive correlation between antioxidant activity and the content of quercetin.The monthly change trends of rutin content and its antioxidant activity were comprehensively analyzed,so it can be concluded that rutin could be used as the main index to evaluate the quality of Folium Hibiscus.

Key words: Folium Hibiscus, flavonoids, antioxidant, monthly periodicity, UFLC