In order to explore the appropriate light quality to simultaneously improve multiple bioactive compounds productivities, the synergistic effects of white light(400-700 nm), red light(620-650 nm) and green light(500-580 nm) on the microalgal growth and bioactive compounds biosynthesis of Isochrysis sp.ISO-FJ were investigated.The results showed that Isochrysis sp. ISO-FJ gave the highest biomass in the white light.In term of the bioactive compounds, the green light was more beneficial to the coproduction of fucoxanthin, exopolysaccharides and polyunsaturated fatty acids in microalgal cells, in comparison to the white or red light.These results together suggested that the growth and bioactive compounds biosynthesis of Isochrysis sp. were regulated by light qualities, which could offer practical basis and theoretical guidance for the directed regulation of microalgal growth and bioactive compounds by marine microalgae Isochrysis strains.