Abstract:

The aim of this study was to investigate the relationship between the alkaloid and polysaccharide content of Fritillariae Ussuriensis Bulbus and various ecological factors,while also evaluating its in vitro antioxidant activity.Samples of Fritillariae Ussuriensis Bulbus were collected from six different regions to analyze the alkaloid and polysaccharide content,along with measurements of soil fertility and relevant ecological factors.Additionally,the antioxidant effects were assessed using the DPPH radical scavenging method,superoxide anion radical scavenging method,and total reducing power assay.The results revealed significant regional differences in alkaloid (0.10%-0.81%) and polysaccharide (5.24%-19.21%) content.Key factors,such as annual average temperature,annual precipitation,sunlight hours,and soil levels of potassium and phosphorus,were found to be closely correlated with the content of these components.Alkaloids exhibited notable activity in DPPH radical scavenging and total reducing power,with the highest DPPH scavenging rate reaching 82.53% and the maximum total reducing power at 1.032.Polysaccharides were more effective in scavenging superoxide anions,although they did not significantly impact DPPH radicals.The study suggests that optimizing ecological conditions and increasing phosphorus and potassium fertilization can enhance the accumulation of active compounds in Fritillariae Ussuriensis Bulbus.Moreover,the demonstrated antioxidant potential of alkaloids and polysaccharides provides a theoretical foundation for the scientific cultivation of Fritillariae Ussuriensis Bulbus and its development as an antioxidant resource.

Key words:

Fritillariae Ussuriensis Bulbus, alkaloids;polysaccharides;ecological factors;antioxidant;correlation study