In order to construct cationic nanocarrier with paclitaxel (PTX) and resveratrol (Rev) co-loaded for reversing multidrug resistance activity.The cationic nanocarrier was prepared by the thin-film dispersion method;The prescription of cationic nanocarrier with PTX and Rev co-loaded was optimized by Box-Behnken response surface method;In vitro drug release study was conducted using dialysis method;The reversing multidrug resistance activity was evaluated by the standard MTT assay.The encapsulation efficiency of PTX and Rev in cationic nanocarrier were 98.93% and 81.23%,respectively;The average particle size was 186.33 nm,and the zeta potential was 6.38 mV;The drug-loaded nanocarrier exhibited significant slow-release drug behavior in vitro;The cytotoxicity studies demonstrated that cationic nanocarrier had high cellular inhibition in drug-resistant cells,the reversal of resistance index was 9.05.The cationic nanocarrier with optimal prescription was suitable for the co-delivery of PTX and Rev,achieved excellent reversing multidrug resistance efficacy.