To solve the problems of small loading capacity and complicated separation process of cell membrane chromatography materials,this research prepared L02 steatosis cell membrane modified magnetic carbon nanotube affinity chromatography materials (CM@MCNTs).It was characterized by scanning electron microscopy,X-ray photoelectron spectroscopy and vibrating sample magnetometer.Combined with ultra performance liquid chromatograph (UPLC),it was rapidly screened the active components of Rhei Radix et Rhizoma for regulating blood lipid.Molecular docking technique was used to simulate the action of active components and related targets.The results showed that the surface of CM@MCNTs is covered with cell membrane,and the characteristic peaks of Fe 2p (706 eV) and N 1s (398 eV) appear,and the material is superparamagnetic,indicating that the material has been successfully prepared.UPLC analysis showed that 13 chemical components were specifically adsorbed,respectively:aloe-emodin-8-O-β-D glucopyranoside,chrysophanol-8-O-β-D glucoside,aloe emodin,rhein,emodin,chrysophanol,physcion and six unknown components.The binding activity between the seven known active ingredient and peroxisome proliferators-activated receptors α (PPARα) was greater than that of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMGCR),mainly through hydrogen bonding.Compared with cell membrane chromatography materials,CM@MCNTs has the advantages of large loading capacity and fast separation speed,with the membrane loading capacity increased by 1.5 times and separation time shortened to 1/20.CM@MCNTs can realize rapid screening of active ingredients of Rhei Radix et Rhizoma.The research also provide a new idea for the high throughput screening of active ingredients in the complex system of traditional Chinese medicine.