Abstract:

This study aims to investigate the chemical constituents and anti-inflammatory activity of Xanthoceras sorbifolia seed meal. The n-butanol extract of the ethanol extract of X. sorbifolia seed meal was isolated and purified by recrystallization, Sephadex LH-20 gel column chromatography, silica gel column chromatography and semi-preparative high performance liquid chromatography. The inhibitory effects of the extract and selected compounds on NO production in LPS-induced RAW 264.7 cells were detected using the Griess method. The structures of the obtained compounds were identified by physicochemical properties, nuclear magnetic resonance and mass spectrometry data. Twenty compounds were isolated from the ethanol extract of X. sorbifolia seed meal and identified as catechol (1), ferulic acid (2), caffeic acid (3), ethyl caffeate (4), 4-methoxycinnamic acid ethyl ester (5), bis(2-ethylhexyl) phthalate (6), dibutyl phthalate (7), butylisobutyl phthalate (8), dibutyl terephthalate (9), esculin (10), justicidin A (11), aneglycoside D (12), carbamide (13), 5-octenyl sulfate (14), octacosanol (15), erythritol (16), dilactic acid (17), (Z)-2-butyroxy-8-heptadecene (18), 6, 9-heptadecadiene (19) and sucrose (20). All compounds were isolated from X. sorbifolia for the first time. The results of anti-inflammatory activity in vitro showed that the n-butanol fraction (300 μg/mL) and compounds 8 (200 μmol/L), 10 (200 μmol/L) and 14 (200 μmol/L) could significantly inhibit the release of NO from RAW 264.7 cells induced by lipopolysaccharide, and the inhibition rates were 22.07 %, 17.65 %, 30.98 % and 21.15 %, respectively, with potential anti-inflammatory activity.

Key words: Xanthoceras sorbifolia seed meal, chemical composition, anti-inflammatory activity, macrophage RAW 264.7