This study investigated the composition content and main chain monosaccharide composition characteristics of the water-soluble polysaccharide of Lycii Fructus (LFWP). Furthermore, it evaluates the intervention effect of LFWP on intestinal inflammation induced by stress in mice subjected to a confined space environment. The LFWP was analyzed using methods such as partial acid hydrolysis, pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP), high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, and scanning electron microscopy. The effect of LFWP on intestinal inflammation was assessed using HE staining and ELISA method. Compositional and structural studies have shown that LFWP contained 90.5 mg/g of sugar, 10.7 mg/g of uronic acid, and 0.4 mg/g of protein. The main-chain fraction exhibited a homogeneous distribution, with galactose as the predominant monosaccharide. Pharmacological studies have demonstrated that a confined space environment can induce colonic tissue damage and inflammatory cell infiltration in mice, marked by elevated serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 (P < 0.001), and reduced IL-10 (P < 0.001). Colonic tissue levels of lipopolysaccharide (LPS), TNF-α, IL-1β, and IL-6 were also increased (P < 0.001, P < 0.05, P < 0.01), alongside elevated calprotectin (CALP) (P < 0.05) and myeloperoxidase (MPO) activity (P < 0.01), and decreased IL-10 (P < 0.001), indicating confined space environment induced intestinal inflammation. High-dose LFWP intervention significantly reduced serum TNF-α, IL-1β, and IL-6 (P < 0.01, P < 0.001), increased IL-10 (P < 0.001), and lowered colonic LPS, TNF-α, IL-1β, and IL-6 levels (P < 0.001), while restoring IL-10 (P < 0.05), CALP (P < 0.001), and MPO activity (P < 0.01) towards normal. LFWP intervention could reduce the level of inflammation and ameliorate stress injury in the mouse intestine in a confined space environment.

"> Lycii Fructus water-soluble polysaccharide, carbohydrate composition, confined space, inflammatory damage to the intestinal