Abstract: The cytotoxicity of vaccarin on human EA·hy926 endothelial cells was investigated by measuring the release of lactate dehydrogenase (LDH) in extracellular fluid.The model of oxidative injury was induced by hydrogen peroxide (H₂O₂) and the model of high glucose injury was induced by high glucose after treatment of these cells with different doses (13.76,6.88,3.44 μmol/L) of vaccarin and 100 μmol/L of vitamin C.Then the cell viability was measured by SRB method.The release of LDH,the level of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in EA·hy926 were determined according to the manufacturer’s instructions.Hence,the protective effect of vaccarin against H₂O₂ and high glucose induced endothelial cellular damage was confirmed.As shown,the superior effect of vaccarin was observed at 13.76 μmol/L,which significantly reduced the level of MDA and LDH leakage,and enhanced production of SOD.

Key words: vaccarin, human EA·, hy926 endothelial cells, H₂O₂ injury, high glucose injury