Abstract: In order to determinate the content of solanesol in flue-cured tobacco leaves by near-infrared diffuse reflectance technology,the near-infrared quantitative calibration model was established using the method of partial least squares (PLS) based on optimizing the spectral data pretreatment method.The crossvalidation RMSECV of calibration model was 0.108,the external-validation RMSEP of calibration model was 0.0822,the average relative deviation was 5.3%, which indicated solanesol content can be predicted quickly and accurately according to the established model.The near-infrared quantitative calibration model was also transferred to other machines by marking transfer method.This achieved rapid detection of solanesol content in different laboratories.

Key words: near-infrared diffuse reflectance, flue-cured tobacco, solanesol, calibration model