Abstract: Five fractions (petroleum ether fraction,CHCl₃ fraction,EtOAc fraction, n-BuOH fraction and residual fraction) obtained from 95% ethanol extract of Grifola frondosa fruiting bodies were analyzed to identify the chemical components by the system preparative tests and screened for their antitumor and DPP-IV inhibitory activities.The results indicated that compounds such as alkaloids,amino acids or proteins,organic acids,phenols,tannins,steroids and anthraquinone existed in G.frondosa fruiting bodies.Petroleum ether fraction,CHCl₃ fraction,EtOAc fraction and  n-BuOH fraction showed no inhibition to WPMY-1 normal cells but showed inhibition to the proliferations of L1210,SW620,K562 tumor cells in whole or part,especially CHCl₃ fraction showed strong inhibition to the proliferation of L1210 tumor cell at IC₅₀ of 0.13 μg/mL.It indicated that these 4 fractions had antitumor activity.The residual fraction showed inhibition to L1210,K562 tumor cells and WPMY-1 normal cells,which demonstrated the residual fraction had cytotoxicity.Moreover,CHCl₃ fraction,EtOAc fraction and  n-BuOH fraction showed strong inhibitory activity to DPP-IV at IC₅₀ of 497.33,776.51 μg/mL and 704.24 μg/mL respectively.These results indicated that these 3 fractions had probability of hypoglycemic activity.

Key words: Grifola frondosa, fruiting body, antitumor activity, DPP-IV inhibitory activity