NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (Suppl): 26-28. doi: 10.16333/j.1001-6880.2015.S.008

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Isolation and Identification of Sapogenin Component from Rubus parvifolius L. by Preparative HPLC

YU Qi-nan1,2, YANG Rong-ping1, JIA Cheng-you4，ZHOU Wen-jie3, LI Na1*   

  1. 1 Chongqing Academy of Chinese Materia Medica，Chongqing 400065，China; 2 Chongqing Fuling Pharmacauticals Co.Ltd,Taiji Group,Chongqing 408000, China; 3 Chong Qing Drug Research co,LTD.of Tian Sheng Pharmaceutical Group,Chongqing  400060,China; 4 Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
  • Online:2015-12-01 Published:2016-01-12

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Abstract: To establish a preparative high performance liquid chromatography (HPLC) method for the separation of sapogenin component in Rubus parvifolus L..The crude alcohol extract of R.parvifolius was initially purified by macroporous adsorptive resins column chromatography and silica gel column chromatography.Then,the initially purified extract was further purified using preparative HPLC.The purity of isolated compounds was determined by analytical HPLC and their structures were identified by MS and NMR.Two sapogenin components were isolated and identified as euscaphic acid and tormentic acid.Tormentic acid was isolated from this plant for the first time.The developed preparative HPLC method was simple,fast and reliable,which was suitable to be used for the separation of sapogenin component from R.parvifolius.

Key words: Rubus parvifolius L., preparative HPLC, euscaphic acid, tormentic acid

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