NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (Suppl): 151-155. doi: 10.16333/j.1001-6880.2015.S.036

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Optimization of Extraction of Flavonoids from the Leaves of Rhamnus utilis by Response Surface Methodology

GAO Xia1,KONG Dong-mei1*,WEI Xiao-jun2   

  1. 1 College of Life Science, Shanxi University,Taiyuan 030006,China; 2 WeiMing Western Medicine Clinic of Jiaokou County,Lvliang 032400,China
  • Online:2015-12-01 Published:2016-01-12

Abstract: Flavonoids in Rhamnus utilis were extracted with ultrasonic-assisted extraction technology using ethanol as solvent.A series of single-factor experiments were made to investigate the effects of ethanol concentration,liquid/solid ratio,extraction time and temperature on flavonoids yield.Response surface method was used to optimize the extraction process.The optimum extraction parameters were determined as:ethanol concentration of 70%,liquid/solid ratio of 33:1,extraction time of 50 min and temperature of 30 ℃.Under these conditions,the extraction yield of total flavonoids was 35.07 mg/g,which was consistent with the predicted value (35.18 mg/g).

Key words: Rhamnus utilis, flavonoids, extraction, response surface

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