Physical-chemical Properties,Isolation and Identification of Antimicrobial Substances from Pleurotus tuber-regium Fermenting Borth

Abstract

Abstract: In order to look for the antimicrobial substances from Pleurotus tuber-regium fermenting liquor,the effects of temperature,UV,pH changes and protease on the antimicrobial substances from Pleurotus tuber-regium fermenting broth were studied.Then the antimicrobial substances were isolated,purified by silica gel column chromatography and reversed-phase medium-pressure liquid chromatography,and a kind of antimicrobial compound was identified by combining ¹H-NMR and ¹³C-NMR with related papers.The results show that the antimicrobial substances in fermenting liquor are stable when influenced by protease,sensitive to ultraviolet radiation and better activity below 90 ℃ and at pH1-9 Guided by antimicrobial activity,a kind of antimicrobial compound PtrA was isolated and its structure was elucidated as 2-furoic acid.

Key words: fermenting liquor, antimicrobial activity, physical-chemical properties, isolation and identification

CLC Number:

Q936
Q586

LIN Chen-qiang1, YAN Jun2, WANG Yan-lin2, CHEN Ji-chen1, LIN Xin-jian1*. Physical-chemical Properties,Isolation and Identification of Antimicrobial Substances from Pleurotus tuber-regium Fermenting Borth[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(4): 534-538.

[1]	ZOU Jin-lian, ZHU Kui-lin, YIN Rui, DENG Mao-xin, WANG Hai-ming, CHEN Zhang-xian, LI Wei-jiao, DONG Fa-wu. Chemical constituents from EtOAc extract of Angelicae Sinensis Radix and their anti-inflammatory activities [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(9): 1645-1652.
[2]	YU Jing-yi, ZHANG Xue-bin, ZHOU Sheng-tuan, HU Wen-jie. Chemical consitituents of the ethyl acetate extract fraction from leaves of camphor-type Cinnamomum camphora and their inhibitory effects on pathogen of anthracnose in Camellia oleifera [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(8): 1471-1477.
[3]	FAN Xuan-xuan, WEI Sheng-xiang, GAO Xiao-yan, CHEN Jun, HAO Ku-ku, DAI Ya-feng, CHEN Nai-fu, CHEN Nai-dong. Chemical constituents from the n-butanol fraction of Dendrobium huoshanense and their inhibitory effect on human gastric adenocarcinoma AGS cells [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(12): 2267-2273.
[4]	SHANG Ying, ZHU Yi-dong, ZHANG Hui, JIANG Zhi-bo, MA Xiao-li, NAN Ze-dong. Chemical constituents from the ethyl acetate extract of Lycium barbarum L. fruits and their biological activities [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(1): 57-64.
[5]	WEI Kai-xin, SONG Xiong-hui, LIU Xiang-qian, XU Yi, SUO Zong-wu, TANG Si-qi, HUANG Hao, LI Xiao-jun. Phytochemical constituents from Dryopteris Crassirhizomae Rhizoma and their anti-inflammatory activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(1): 65-73.
[6]	CHEN Li, HUANG Sheng, SU Guo-qi, YANG Fei-yun, HUANG Jin-xiu, QI Ren-li, LIU Zuo-hua. Identification and antimicrobial activity of a strain of Paenibacillus elgii [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(9): 1499-1511.
[7]	ZHANG Li, SUN Jian-peng, BA Qi-bin. Chemical constituents from ethyl acetate fraction of Houttuynia cordata and their anti-plant pathogens activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(4): 616-621.
[8]	YU Jing, LI Yi-hang, YIN Cui-yun, DENG Zhao-you, TANG De-ying, ZHANG Li-xia. Diversity and biological activity of endophytic fungi isolated from Amomum villosum Lour. in different regions of Yunnan Province [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(9): 1540-1553.
[9]	LIU Yu, HU Qi, HE Ya-nan, JIANG Yan, WU Jia-ying, YANG Xin, ZHANG Ding-kun, YANG Ming. Screening quality markers for antibacterial activity of Artemisia argyi leaves essential oil based on spectrum-effect relationships [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(6): 1017-1026.
[10]	CHEN Jie, WANG Dan, ZHANG Xiong, PENG Ming-you, XU Ran, YAN Ying, WANG Ying. Study on chemical constituents and antibacterial activity of Carpesium faberi [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(4): 598-605.
[11]	LI Xiao-ju, TUO Cheng-jie, HUANG Zhi-pu, ZHANG Xiao-mei, ZHAO Qing. Study on the antibacterial activity of six gingeroloids from Zingiber officinale [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(2): 220-225.
[12]	XU Jin-ye, XU Feng-qing, KONG Chui-hao, ZHAO Hong-su, WANG Ren-zhong, WU De-ling. Chemical constituents from the roots of Stephania tetrandra and their anti-liver fibrosis activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(12): 2050-2055.
[13]	LIANG Wei-zhong, XIAO Yi-wen, ZHANG Wen-hui, WANG Ya, GAO Bo-liang, ZHU Du. Isolation of secondary metabolites and antimicrobial activity of endophytic fungus Sarocladium oryzae L99 [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2021, 33(增刊1): 35-42.
[14]	PENG Fang, XIONG Liang, HE Yu-lin, LIU Fei, PENG Cheng. 8-O-4′ Neolignans from the fruits of Leonurus japonicus [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2021, 33(8): 1320-1325.
[15]	LIU Wei, REN Jia-li, LI Xin-yu, CHEN Lin, HUANG Shuai. Diterpenoid alkaloids from the roots of Aconitum forrestii Stapf [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2021, 33(3): 419-425.

Physical-chemical Properties,Isolation and Identification of Antimicrobial Substances from Pleurotus tuber-regium Fermenting Borth

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments