Abstract: In this study,the content of main effective ingredients including puerarin,daidzin and total flavonoids of Pueraria lobata from 5 areas of Shanxi were investigated by HPLC. In addition,the HPLC analysis conditions were optimized.The results showed that:there was a significant difference in the content of puerarin,daidzin and total flavonoids of 6 samples of P.lobata. The contents of puerarin,daidzin and total flavonoids were 1.46%~4.48%,0.11%~0.62% and 11.19%~26.14%,respectively.Puerarin and total flavonoids had positive correlation with the cultivation latitude of P.lobata, The contents of puerarin in P.lobata samples from Xiaobei Mountain,Meli town,Jiangxian and Taikuan river Nature Reserve,Sijiao town,Xiaxian were not match up with the standard (2.4%) required by Chinese Pharmacopoeia,while the content of puerarin from Panjiapu,Xiaguan,Lingqiu was determined to be 4.48%.

Key words: Pueraria lobata, germplasm resources, daidzin, puerarin, total flavonoids