Ten compounds were separated and purified from ethyl acetate fraction derived from 95% EtOH extract of Houttuynia cordata by silica gel,macroporous resin,Sephadex LH20,MCI resin,thin-layer chromatography and high performance liquid chromatography.Their structures were identified on the basis of mass spectrometry(MS),nuclear magnetic resonance and comparison with the data in reported literatures. As a result,ten compounds were identified as dehydrovomifoliol (1),5,6-epoxy-3-hydroxy- 7-megastigmen-9-one (2),loliolide (3),pubinernoid A (4),quercitrin (5),quercetin (6),afzelin (7),4-methoxybenzene-1,2-diol (8),4-hydroxybenzaldehyde (9),benzoic acid (10).Compounds 1-4 were norsesquiterpenoids isolated from Houttuynia cordata for the first time. All the compounds were screened for their anti-plant pathogens activities,and at the concentration of 40 mg/mL,the inhibition rates of compounds 5-7 ranged from 38% to 50% against Fusarium graminearum, Fulria fulva,Botrytis cinerea,Mycosphaerlla melonis and Fusarium oxysporum f.sp. vasinfectum.