NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2011, Vol. 23 ›› Issue (1): 110-113.

• Article • Previous Articles     Next Articles

Preparative Separation of Flavones from Peanut Hull by High-Speed Counter-Current Chromatography

NIU Dan-dan1,2, LIU Cai-xia1,2, LIU Xiu-hua2, LI Ming-jing1,2*   

  1. 1College of Chemistry and Chemical Engineering; 2Key Laboratory of Natural Medicine and Immune Engineering, Henan Province, Kaifeng 475004, China
  • Received:2009-10-20 Published:2011-07-20

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Abstract:

Three flavones were prepared, isolated and purified from peanut hull by high-speed counter-current chromatography (HSCCC) for the first time. A two-phase solvent system composed of n-hexane : ethyl acetate : methanol : water : acetic acid (5:3:3.5:5:0.25, v/v) was used. The obtained fractions were analyzed by HPLC and identified by MS, 1H and 13C NMR. Within 100 minute, 11.0 mg of luteolin, 2.2 mg of diosmetin, and 5.2 mg of 5,7-dihydroxychromone with their purities over 96.0% were obtained from 70 mg crude extract of peanut hull in one-step elution under the conditions of a flow rate of 2.0 mL/min, while the apparatus rotated at 800 r/min and the effluent was detected at 275 nm. The results indicated that HSCCC was a powerful technique for the separation and purification of flavones from the peanut hull.

Key words: HSCCC, peanut hull, Luteolin, Diosmetin, 5,7-dihydroxychromone