In order to study the chemical constituents from Dryopteris championii,the isolation and purification of the 95% EtOH extract of the aboveground part of D. championii were carried out with various chromatographic separation techniques of silica gel,ODS,Sephadex LH-20,as well as the preparative high performance liquid chromatography.It led to the isolation of thirteen flavonoids compounds of which structures were determined by spectral analysis and comparison with literature data.They were identified as (-)-epicatedchin (1),luteolin (2),naringenin (3),eriodictyol (4),quercetin (5),apigenin (6),kaempferol (7),astragalin (8),avicularin (9),quercetin-3-O-β-D-glucopyranoside (10),vitexin (11),apigenin-7-O-β-D-glucopyranoside (12),and quercetin-3-O-6-E-p-coumaroyl-β-D-glucopyranosyl-(1→2)-α-L-rhamnopyranoside (13),including one flavanol,two flavanones,four flavones,and six flavonols. Among them,all of the compounds were isolated from this plant for the first time,and of which compounds 6,8,9,11 and 13 were obtained from the genus of Dryopteris for the first time.