To systematically investigate the chemical constituents of mango kernels,ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) was analyzed in this study.Electrospray ion source (ESI) was adopted to collect the data in negative ion mode.A total 135 compounds were identified according to analyze the mass data from the references and related literatures,including 47 berberine-type alkaloids,47 flavonoids,30 organic acids and three coumarins,eight iridoid glycosides.Seventeen compounds were identified in this genus for the first time.Using UPLC-Q-TOF-MS/MS technology to analyze the chemical constituents of mango kernels,which will provide a significant basis for the subsequent study of material basis and clinical application.