天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (2): 328-332.doi: 10.16333/j.1001-6880.2015.02.026

• 开发研究 • 上一篇    下一篇

基于微量筛选模型的48种中草药提取物乙酰胆碱酯酶抑制活性筛选及评价

周思多1,2，王晓2，冯金红2，耿岩玲2，赵恒强2*   

  1. 1 山东农业大学食品科学与工程学院，泰安 271018；2 山东省科学院中药过程控制研究中心山东省大型精密分析仪器应用技术重点实验室 山东省分析测试中心，济南 250014
  • 出版日期:2015-02-15 发布日期:2015-02-15

Screening and Evaluation of Acetylcholinesterase Inhibitory Activity Based on Microscale Screening Model of 48 Traditional Chinese Medicines

ZHOU Si-duo1,2, WANG Xiao2, FENG Jin-hong2, GENG Yan-ling2, ZHAO Heng-qiang2*   

  1. 1 College of Food Science and Engineering,Shandong Agricultural University,Shandong Academy of Sciences, Tai’an 271018,China;2 Process Control Research Center of Traditional Chinese Medicine of Shandong Academy of  Sciences,Shandong Analysis and Test Center,Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province,Shandong Academy of Sciences,Jinan 250014,China
  • Online:2015-02-15 Published:2015-02-15

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摘要： 采用微量筛选模型考察48种中药提取物的乙酰胆碱酯酶抑制活性。采用不同极性溶剂对中药材进行提取，采用微量筛选模型对不同浓度提取物的乙酰胆碱酯酶抑制活性进行考察，并采用Origin8.0计算其IC50值。结果表明，几种中药材的不同极性溶剂（水、80%乙醇、乙酸乙酯、石油醚）提取物有不同程度的乙酰胆碱酯酶抑制活性。其中黄柏、元胡的80%乙醇提取物，丹参的乙酸乙酯提取物的抑制率较高。当上述提取物在实验体系中的终浓度为76.92 mg/L时，抑制率分别为66.72±0.02%、52.67±0.04%、46.84±0.03%，其IC50值分别为295.12、229.09、1202.26 mg/L。本研究结果为从中草药中快速筛选乙酰胆碱酯酶抑制剂提供了方法和数据支持。

关键词: 中药, 乙酰胆碱酯酶, 微量筛选模型

Abstract: In this paper,the acetylcholinesterase (AChE) inhibitory activities of 48 traditional Chinese medicine extracts were investigated by microscale screening model.Different polar solvents such as distilled water,80% ethanol (v/v),ethyl acetate and petroleum ether,were used as extraction solvents,the AChE inhibitory activities of these extracts were investigated by microscale screening model,and the IC50 values were evaluated by Origin 8.0.The results showed that some of the extracts derived from the 48 traditional Chinese medicines had AChE inhibitory activities,in which the inhibitory rates of the 80% ethanol extracts of Cortex Phellodendri,Rhizoma Corydalis,and ethyl acetate extract of Salvia miltiorrhiza were the highest.When the final concentration of the extracts in the experimental system were 76.92 mg/L,the inhibitory rates of the 3 extracts were 66.72±0.02%,52.67±0.04% and 46.84±0.03%,respectively.The IC50 values were 295.12,229.09 mg/L and 1202.26 mg/L,respectively.This study provided method and data support for rapid screening of AChE inhibitors from traditional Chinese medicines.

Key words: traditional Chinese medicine, acetylcholinesterase, microscale screening model

中图分类号: 

R285.5

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