天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (7): 1237-1241.doi: 10.16333/j.1001-6880.2015.07.021

• 开发研究 • 上一篇    下一篇

翼蓼块根提取物的抗氧化活性和血管紧张素转化酶抑制活性研究

王兰1，郭利杰1，许明录2*   

  1. 1 河南师范大学，新乡 453007；2 河南科技学院，新乡 453003
  • 出版日期:2015-07-20 发布日期:2015-07-23

Antioxidant and ACE Inhibition Activity of Different Extracts of Pteroxygonum giraldii Root

WANG Lan 1,GUO Li-jie 1,XU Ming-lu 2*   

  1. 1 College of Life Science,Henan Normal University,Xinxiang 453007,China;  2 Henan Institute of Science and Technology,Xinxiang 453003,China
  • Online:2015-07-20 Published:2015-07-23

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摘要： 本文主要对翼蓼块根的甲醇提取物及其乙酸乙酯、正丁醇、水萃取层的DPPH自由基清除能力、还原力、金属离子螯合能力和ACE抑制活性进行研究。四种提取物的总酚含量分别为147.97、228.31、162.99、168.16 mg没食子酸/g，黄酮含量分别为19.45、21.57、13.89、14.80 mg槲皮素/g。DPPH自由基清除能力的EC50值分别为52.37、11.43、46.45、50.32 μg/mL。四种提取物的还原能力均表现出随着浓度增加，其还原能力也增加的趋势；当终浓度为100 μg/mL时，各提取物还原能力均强于抗氧化剂BHA。在100 μg/mL时，乙酸乙酯层具有最强的金属螯合能力。在ACE抑制实验中，甲醇提取物和乙酸乙酯层在100 μg/mL时,抑制率分别为39.45%和39.76%。实验结果表明，翼蓼块根的总酚和总黄酮含量与其抗氧化活性呈正相关。抗氧化活性与其对应的ACE抑制活性存在一定程度的联系，具体发挥ACE抑制活性的单体化合物尚需要进一步分离分析研究。

关键词: 翼蓼, 总酚, 总黄酮, 抗氧化活性, ACE

Abstract: In this study,the methanol extract of Pteroxygonum giraldii root and its ethyl acetate fraction,butanol fraction and water fraction were used to investigate the DPPH radicals scavenging activity,reducing power,metal chelating and angiotensin converting enzyme (ACE) inhibition activity.The total phenol content of four extracts were 147.97,228.31,162.99,168.16 mg gallic acid/g,the total flavonoid content of four extracts were 19.45,21.57,13.89,14.80 mg quercetin/g respectively,the four extract exhibited strong DPPH free radical scavenging activity with the EC50 of 52.37,11.43,46.45,50.32 μg/mL respectively.The reducing power of all samples showed a dose-dependent manner.The reducing power was even stronger than the standard BHA at concentration level of 100 μg/mL.The ethyl acetate fraction showed 37.79% metal chelating ability at 100 μg/mL.The methanol extract and ethyl acetate fraction exhibited the 39.45% and 39.76% ACE inhibition activity at 100 μg/mL.These results showed that the antioxidant activities of P.giraldii extract were correlated with its total phenolic and flavonoid contents.In addition,there was a positive correlation between the antioxidant activity and ACE inhibition activity.

Key words: Pteroxygonum giraldii, total phenol, flavonoids, antioxidant activity, ACE

中图分类号: 

R93

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