天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (9): 1414-1421.doi: 10.16333/j.1001-6880.2016.9.013

• 研究简报 • 上一篇    下一篇

玄参芦头片对玄参药材整体质量的影响研究

许一鸣1，吴啟南1,2,3*，乐巍1，蒋征1，桑梦如1，吴达维1   

  1. 1 南京中医药大学药学院；2 江苏省中药资源产业化过程协同创新中心；3 中药资源产业化与方剂创新药物国家地方联合工程研究中心，南京 210023
  • 出版日期:2016-09-30 发布日期:2017-03-24

Impact of Scrophulariae Rhizome on Overall Quality of Scrophularia ningpoensis

XU Yi-ming1,WU Qi-nan1,2,3*,YUE Wei1,JIANG Zheng1,SANG Meng-ru1,WU Da-wei1   

  1. 1 College of pharmacy,Nanjing University of Chinese Medicine; 2 Collaborative Innovation Center of Chinese Medicinal Resources Industrialization; 3National and Local Collaborative Engineering Center  of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing 210023,China
  • Online:2016-09-30 Published:2017-03-24

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摘要： 本文主要利用高效液相色谱法对玄参芦头片和根片中5种成分（哈巴苷、麦角甾苷、安格洛苷C、哈巴俄苷、肉桂酸）进行含量测定，以探讨玄参芦头片对玄参药材整体质量的影响。首先，采用高效液相色谱法，在210、280、330 nm下对样品进行检测。其次，采用紫外分光光度法，在517 nm下对样品提取液的DPPH自由基清除率进行检测。玄参中所测定的5种成分均达到基线分离，其芦头片与根片提取液的DPPH自由基清除率无显著差异。各批玄参饮片中普遍存在芦头片，实验结果表明玄参芦头片和根片中的哈巴苷和哈巴俄苷总含量差异较小，因此芦头片对玄参药材整体质量基本没有影响。

关键词: 玄参根片, 玄参芦头片, HPLC, 含量测定, DDPH自由基

Abstract: A high performance liquid chromatograph (HPLC) method was established for the simultaneous determination of five components (Harpagide, Verbascoside,Angoroside-C,Harpagoside,Cinnamic acid) in Scrophulariae Rhizome and Scrophulariae Radix,in order to investigate the impact of Scrophulariae Rhizome on the overall quality of Scrophulariae Radix.The determination was performed on a Waters 2695 HPLC with a 2998 DAD detector under 210,280 nm and 330 nm.In addition,the DPPH free radical scavenging rates of Scrophulariae Radix were determined at 517 nm by Ultraviolet Spectrophotometry.Consequently,the five components reached the baseline separation and there were no significant differences in the DPPH free radical scavenging rates between the extracts of Scrophulariae Rhizome and Scrophulariae Radix.Each batch of Scrophulariae Radix generally contained Scrophulariae Rhizome,and the total contents of Harpagide and Harpagoside in Scrophulariae Rhizome was similar to those in Scrophulariae Radix.Therefore,the existence of Scrophulariae Rhizome had little impact on the overall quality of Scrophulariae Radix.

Key words: Scrophulariae Radix, Scrophulariae Rhizome, HPLC, content determination, DPPH free radical

中图分类号: 

R932

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