关键词: 岩大戟内酯A, 岩大戟内酯B, MCF-7细胞, 细胞增殖, Akt信号通路

Abstract: To observe the effects of JolkinolideA (JA) and Jolkinolide B (JB) on the proliferation and apoptosis of MCF-7 human breast cancer cells and analyze the mechanism of JA or JB.In this experiment,40,60 and 80 μg/mL of JA or JB were used to stimulate MCF-7 cells,respectively.The cells stimulated by the different concentrations of JA or JB were used as the JA groups or JB groups,respectively.The normal cultured MCF-7 cells were used as the control group.CCK-8 assay was used to detect the OD value of cell proliferation in JA groups and JB groups;Western blot experiment was used to detect the expression of Caspase 9 protein in JA groups and JB groups;Annexin V-FITC/PI experiment was used to detect the apoptosis rate of MCF-7 cells in JA and JB groups;Enzymelinked immunosorbent assay (ELISA) experiment was used to detect the contents of Akt and STAT3 protein in JA groups and JB groups.The experimental results found that compared with the JA groups (40,60 and 80 μg/mL),the OD values of MCF-7 cell proliferation in the JB groups with the corresponding concentrations were significantly decreased,and the relative absorbance values of Caspase 9 protein and the cell apoptosis rates in JB groups were significantly increased;Compared with the JA groups,the contents of VEGF in the supernatants of JB groups with the corresponding concentrations were decreased,and the expression of Akt and STAT3 protein were also decreased.These results indicated that JB can promote the apoptosis and inhibit the proliferation of MCF-7 cells by inhibiting the Akt-STAT3 signaling pathway.

Key words: Jolkinolide A, Jolkinolide B, MCF-7 cells, cell proliferation, Akt signaling pathway