关键词: 淡紫拟青霉菌, 灰毛豆, 内生真菌, 抑菌活性

Abstract: Purureocillium lilacinum (TPL04) is an endophytic fungus isolated from the leaves of Tephrosia purpurea.To investigate the secondary metabolites of the strain (TPL04),the chemical constituents of the fermentation products were isolated by repeated silica-gel column chromatography,Sephadex LH-20,preparative thin layer chromatography,as well as medium pressure column chromatography and preparative HPLC.Finally,eight compounds were obtained,and their structures were elucidated as 5-hydroxyhexan-4-olide (1),(R)-4-benzyl-2-oxazolidinone (2),2′-deoxythymidine (3),Methylparaben (4),Lumichrome (5),β-Daucosterin (6),Uridine (7),Hexitol (8),respectively,by spectroscopic methods.Among them,compounds 1,2,and 4 were isolated from P.lilacinum for the first time.The antifungal test showed that compound 4 could inhibit the growth of four plant pathogens,Pellicularia sasakii,Phytophthora capsici,Botrytis cinerea,and Sclerotinia sclerotiorum.

Key words: Purureocillium lilacinum, Tephrosia purpurea, endophytic fungi, antifungal activity