关键词: 茶多酚, 表没食子儿茶素没食子酸酯, 纯化, 吸附柱层析

Abstract: Adsorption resin chromatography was applied to purify (-)-epigallocatechin gallate(EGCG) monomer from tea polyphenols raw material.LX-8 was used for the preliminary purification and HP-20 resin was used for further purification according to adsorption capacity,adsorption ratio,desorption ratio and adsorption selectivity coefficient of the resins chosen towards catechins.After the second run of purification with LX-8 fed with 25% (v/v) ethanol aqueous product,a product with an EGCG purity of 73.06% was obtained after eluted by 40% (v/v) ethanol solution at a flow rate of 1.5 BV/h.Product of LX-8 40% (v/v) ethanol aqueous with 20 mg/mL concentration being raw material,the second column packed with HP20 was eluted with 3 BV,6 BV and 3 BV ethanol solutions at increasing concentrations (5,15 and 80%,v/v) at a flow rate of 1.5 BV/h,respectively.After eluted by different aqueous ethanol,a product with an EGCG purity of 93.16% and an overall recovery of 49.50% was achieved.

Key words: tea polyphenols, (-)-epigallocatechin gallate, purification, adsorption resin chromatography