天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (Suppl1): 56-59.

• 研究简报 • 上一篇    下一篇

普洱熟茶发酵过程多酚、咖啡碱以及茶色素的变化研究

李家华1,倪婷婷1,张广辉1,袁文侠1,邵婉芳1,桥本文雄2,赵明1*   

  1. 1 云南农业大学龙润普洱茶学院,昆明 650201;2 鹿儿岛大学农学院园艺学系,鹿儿岛 8900065
  • 出版日期:2014-12-30 发布日期:2015-03-04

Content Variation of Polyphenols,Caffeine and Pigment during Fermentation of Pu-erh Shucha

LI Jia-hua 1, NI Ting-ting1, ZHANG Guang-hui1, YUAN Wen-xia1, SHAO Wan-fang1, Fumio Hashimoto2, ZHAO Ming 1*   

  1. 1 College of Pu-erh Tea,Yunnan Agricultural University,Kunming 650201,China; 2 Department of Horticultural Science,Faculty of Agriculture,Kagoshima University, Kagoshima 8900065,Japan
  • Online:2014-12-30 Published:2015-03-04

摘要: 后发酵是云南地理标志产品普洱熟茶品质形成的关键,但该过程的化学物质变化仍需研究。本文应用高效液相色谱分析发现随着发酵进行包括表儿茶素[()epicatechin,EC)]、表没食子儿茶素[(-)-epigallocatechin,EGC)]、表儿茶素没食子酸酯[(-)-epicatechin-3-O-gallate,ECG]、表没食子儿茶素没食子酸酯[(-)-epigallocatechin-3-O-gallate,EGCG]在内的儿茶素含量以及酚酸类物质茶没食子素(theogallin,TG)和水解单宁类物质strictinin(STR)、1,4,6-tri-O-galloyl-β-D-glucose(1,4,6-tri-G-G)的含量降低;与之相反,没食子酸(GA)和咖啡碱(CAF)的含量增加。另外在发酵样品中未检测到与红茶样品相同的茶色素,表明红茶与普洱熟茶的色素不同。

关键词: 普洱熟茶, 多酚, 咖啡因, 色素, 发酵

Abstract: Pu-erh shucha,a traditional beverage in Yunnan Province,China,is popular in East Asia.The key process for the development of characteristic flavors of Pu-erh shucha is “post-fermentation”;however,little is known about the content variation of chemical components during this process.In this work,the content variation of tea polyphenols,caffeine and pigments during the fermentation process of Puerh shucha was monitored by high performance liquid chromatography (HPLC).The HPLC analysis revealed that the concentrations of (-)-epicatechin (EC),(-)-epigallocatechin (EGC),(-)-epicatechin-3-O-gallate (ECG),(-)-epigallocatechin-3-O-gallate (EGCG),theogallin (TG),strictinin (STR) and 1,4,6-tri-O-galloyl-β-D-glucose (1,4,6-tri-G-G) decreased,while the concentrations of gallic acid (GA) and caffeine (CAF) increased during the fermentation process.It was inferred that gallate catechins hydrolyze to GA during the fermentation process.In addition,the fact that no black tea pigments were detected in the fermenting samples of pu-erh shucha suggested that different pigments existed in the different teas.

Key words: Pu-erh shucha, polyphenols, caffeine, pigment, fermentation

中图分类号: 

T272