关键词: 红腺忍冬, 叶, 绿原酸, 大孔树脂, 分离纯化

Abstract: Chlorogenic acid was extracted from Lonicera hypoglauca Miq leaf with ethanolheat reflux methods and separated and purified by macroporous resin method,D101 is as the optimum macroporous resin by screening.studies was made on separation and purification of chiorogenic acid from Lonicera hypoglauca Miq leaf extraction by macroporous resin for screening the optimal conditions.UV maximum absorption of chlorogenic acid is used as an indicator to test wavelength at 320nm.The optimum adsorption technology is as follows：resinliquid ratio as 1 g:6 mL，adsorption solution pH 2，adsorption solution flow rate of 60/min .The optimum elution technology is as follows：elution solution as 20% ethanol，volume of elution solution is 30 mL， the flow rate was 40/min， elution solution pH7.Under optimal conditions of separation and purification， average recovery rate and purity of chlorogenic acid from Lonicera hypoglauca Miq leaf extraction is 1.403% and 45.77% respectively.The method developed in the paper has the advantages of simple operation,short production cycle and low cost which appropriate for the separation and purification of chiorogenic acid from Lonicera hypoglauca Miq leaf extraction and industry scale production.

Key words: Lonicera hypoglauca Miq, leaf, chlorogenic acid, D101 macroporous resin, separation and purification