天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (10): 1737-1746.doi: 10.16333/j.1001-6880.2020.10.015

• 开发研究 • 上一篇    下一篇

基于“甾酮类化学成分-抗氧化生物活性”关联分析评价牛膝药材商品等级

常爱冰1，刘妍如1*，王梅2*，唐志书1，宋忠兴1，史鑫波1，刘峰3，陈彦斌3   

  1. 1陕西中医药大学 陕西省中药资源产业化协同中心 省部共建协同秦药特色资源研究与开发重点实验室（培育）陕西省创新药物研究中心；2陕西中医药大学附属医院，咸阳 712083； 3陕西步长制药有限公司，西安 710075

  • 出版日期:2020-10-28 发布日期:2020-10-30
  • 基金资助:
    陕西省科技厅重点产业链（群）项目（2020ZDLSF05-08）；陕西省高层次人才特殊支持计划&青年拔尖人才支持计划（陕组通字［2018］33号）；国家自然科学基金（81773919）；陕西省技术创新引导专项（2018HJCG-21）

Commercial grade evaluation of Achyranthes bidentata based on "phytoecdysones components-antioxidant biological activity" correlation

CHANG Ai-bing1,LIU Yan-ru1*,WANG Mei2*,TANG Zhi-shu1,SONG Zhong-xing1,SHI Xin-bo1,LIU Feng3,CHEN Yan-bin3   

  1. 1Shaanxi University of Chinese Medicine,Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources  Industrialization, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources（Cultivation), Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research,Xianyang 712083,China; 2The Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712083,China; 3Shaanxi Buchang Pharmaceutical Co.,Ltd.,Xi’an 710075,China

  • Online:2020-10-28 Published:2020-10-30

PDF (PC)

124

摘要：

本研究采用ACQUITY UPLC BEHC18（50 mm × 2.1 mm，1.7 μm）色谱柱，以0.2%乙酸水溶液（A）-乙腈溶液（B）为流动相进行梯度洗脱，体积流量为0.3 mL/min，检测波长为250 nm，在该色谱条件下测定21批牛膝药材甾酮类成分含量。并关联生物活性项下的ABTS自由基清除率、DPPH自由基清除率和羟基自由基清除率的结果，最后建立用于等级评价的Logistic回归模型。通过该模型，21批的牛膝药材被分成了优良中差四个等级。研究结果表明，基于二分类Logistic回归模型描述牛膝药材等级与影响因素之间的映射关系是可行的，为牛膝药材的质量评价标准的制定提供了新的思路。

关键词: 牛膝药材, Logistic回归, 含量测定, 生物活性, 质量控制, 等级评价

Abstract:

The UPLC chromatographic column was acquity ACQUITY UPLC BEHC18 (50 mm×2.1 mm,1.7 μm),with 0.2% acetic acid-acetonitrile as the mobile phases in gradient solvent system.The detection wavelength was set at 250 nm with flow rate of 0.3 mL/min,and phytoecdysones components were determined in the chromatographic conditions.Then the antioxidant biological activity was reflected through ABTS radical scavenging rate,DPPH radical scavenging rate and hydroxyl radical scavenging rate.Finally,a two-classification logistic regression model was based on correlating biological activity and components.All 21 batches of Achyranthes bidentata were divided into four grades according to this model.The results showed that it was feasible to describe the mapping relationship between the grade and the influencing factors by this model,which could provide a new idea for the evaluation standard of A. bidentata.

Key words: Achyranthes bidentata, Logistic regression, content determination, biological activity, quality control, grade evaluation

中图分类号:  R282

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：