NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (3): 393-397.doi: 10.16333/j.1001-6880.2015.03.005

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Extraction of β-Carotene from Spirulina Using Ethanol and Acetone-Ammonium Sulphate Aqueous Two-Phase System

ZHANG Xi-feng1, YANG Sheng-hui2, LUO Guang-hong2*, WANG Dan-xia2   

  1. 1 The College of Agriculture and Biotechnology (CAB),Hexi University;2 Microalgae Engineering  Research Center of Gansu Province,Kaiyuan Bio-tech Development Center,Hexi University,Zhangye 734000,China
  • Online:2015-03-30 Published:2015-04-07

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Abstract: In this study,an aqueous two-phase system composed of 15% (W/W) ethanol and acetone (v/v=1:2) 24% (W/W) ammonium sulphate was selected to extract β-carotene from Spirulina. The effect of crude extract concentration,pH,extraction temperature on the extraction efficiency of  β-carotene were investigated by single factor and Box-Behnken design methods.The results showed that the optimal conditions for the extraction of β-carotene were:crude extract concentration of 6% (W/W),pH of 8.0 and extraction temperature of 30 ℃.The maximal extraction efficiency (94.55%) was obtained under the optimal conditions.These results suggested that ATPE is efficient in extracting β-carotene and has the potential to be used in natural β-carotene extraction industry.

Key words: Spirulina, β-carotene;aqueous two-phase

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