Abstract: In order to explore the accumulation of glucosides in Bozhou Peony Paeonia lactiflora with different cultivation ages and to provide references for the standardized cultivation and quality evaluation of Bozhou Peony,a HPLC-DAD method was developed and applied for the simultaneous determination of paeoniflorin and albiflorin in P.lactiflora. A Lanbo Service 4000 system was used with Waters Symmetry C₁₈ column (4.6mm×150mm,5μm) at 30 ℃ with a gradient elution of acetonitrile and 0.1% phosphonic acid.The flow rate was 1.0 mL/min and the detection wavelength was 230 nm.The calibration curves of paeoniflorin and albiflorin were linear over the ranges of 0.1250-2.5000 μg and 0.0225-0.4500 μg.The average recoveries (n=6) were 100.56% and 101.30%,respectively.The developed method was successfully applied for the analysis of Bozhou Peony P.lactiflora of different plant ages from its Geo-authentic habitats Bozhou region,Anhui province,China.The results also indicated that remarkable variation of the contents of paeoniflorin and albiflorin were existed in Bozhou Peony with different plant ages.In our experimental conditions,the highest contents of paeoniflorin and albiflorin were the Bozhou Peony cultivated for 3 years and 2 years,respectively.Our research results provided some references for the clinical medication of Bozhou Peony.

Key words: Bozhou Peony Paeonia lactiflora, paeoniflorin;albiflorin;HPLC