Abstract: In order to evaluate the subacute toxicity of carboxymethyl chitosan-zinc, thirty Kunming mice,half male and half female,were divided into three groups:experimental group 1,experimental group 2 and control group.The saline maceration extract was made from two kinds of carboxymethyl chitosan-zinc complexes with different content of zinc ions.Mice of experimental groups were given maceration extract by intraperitoneal injection for 28 days,while control group mice were given the same daily amount of normal saline.After that,the weight,related blood biochemical indexes and viscera indexes of mice were measured and pathological examination of organs was done.The results showed that the basic physiological state of all mice was normal in the experiment,but at the end of experiment,the serum content of ALP and UA in the male mice of experimental group 1 was significant lower than that of control group (P<0.05);the serum content of UA in the male mice of experimental group 2 were significant lower than that of control group (P<0.05);no significant statistical difference was detected on other serum biochemical indexes between experimental groups and control group;the renal index of female mice in experimental group 2 was significant higher than that of control group (P<0.05),while other visceral indexes of experimental groups mice were normal,coupled with no significant statistical difference between experimental groups and control group;through the visual observation and pathological observation of heart,liver,spleen,lung,kidney and thymus,no obvious lesions were found,showing that the two kinds of carboxymethyl chitosan-zinc complexes had no evident toxic effects on mice.

Key words: carboxymethyl chitosan-zinc, subacute, abdominal cavity, blood biochemical indicators