NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (12): 2079-2083.doi: 10.16333/j.1001-6880.2015.12.016

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Determination of Nucleosides and Nucleobases in Different Harvesting Periods of Scrophulariae Radix

WANG Sheng-nan1, HUA Yu-jiao1, ZOU Li-si1, LUO Yi-yuan1, LIU Xun-hong1*, LIU Juan-xiu1, YAN Ying1, XU Li2   

  1. 1 Nanjing University of Chinese Medicine,Nanjing 210023,China; 2 Yangzhou Institute for Drug Control,Yangzhou 225000,China
  • Online:2015-12-29 Published:2016-01-06

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Abstract: The goal of this research was to establish an UPLC-QTRAP-MS/MS method for the determination of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix and to investigate the dynamic accumulation of nucleosides and nucleobases in different harvesting periods of Scrophulariae Radix.UPLC-QTRAP-MS/MS was applied for the analysis of 10 kinds of nucleosides and nucleobases in Scrophulariae Radix.Compared with other nucleosides and nucleobases in Scrophulariae Radix,the contents of guanosine,uridine,adenosine and uracil were relatively higher.There were differences in the contents of nucleosides and nucleobases in Scrophulariae Radix at different harvesting periods;the contents were relatively higher in those grown in November.This study provided a scientific basis for the investigation of the influence of the quality formation mechanism of Scrophulariae Radix as well as the determination of the appropriate harvesting time.

Key words: Scrophulariae Radix, UPLC-QTRAP-MS/MS, nucleosides and nucleobases, dynamic accumulation, different harvest periods

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