Abstract: In this paper,the contents and accumulation of total flavonoids in the leaves,flower and fruit of Evodia lenticellata Huang were determined by ultravioletvisible spectrophotometer with colorimetric method.Rutin had good linearity within the range of 7.76-77.60 μg/mL,Y=0.0087X+0.0268 (R²=0.9964).The average recovery was 101.72% with RSD of 2.25% (n=6).The results showed that the content of total flavonoids increased constantly with the growth of E.lenticellata and reached the highest when the fruits was close to maturity.The flower and fruit of E.lenticellata were rich in total flavonoids and can be used as a resource to extract flavonoids.

Key words: Evodia lenticellata Huang, total flavonoids, accumulation