Abstract: The aim of this study was to investigate the in vitro anti-thrombolytic effect and to preliminary evaluate on the bio-security of the fibrinolytic enzyme SNFE from Sipuculus nudus Linnaeus in Guangxi Coastal Area of China.The in vitro anti-thrombolytic effect and action mode of SNFE were elucidated using the plasminogen free and rich wells fibrin plate assay,detecting the residual of thrombin clottable fibrinogen contents,and thrombolytic experiment.The bio-security was assessed by subcutaneous hemorrhage and hemolysis experiments.Results showed that SNFE not only had the kinase activity,but also directly dissolved fibrin and fibrinogen,it can induce clot lysis in vitro, and had no obvious hemolysis and hemorrhagic effects.

Key words: anti-thrombolytic effect, fibrinolytic enzyme, Sipuculus nudus Linnaeu