NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2017, Vol. 29 ›› Issue (10): 1700-1705.doi: 10.16333/j.1001-6880.2017.10.012

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Metabolism of Active Fractions of Inula Cappa in Rat Bile

GONG Zi-peng* ,HOU Jing-yu, HOU Jia, WU Ping, LI Yue-ting, CHEN Shi-ying, LAN Yan-yu   

  1. Guizhou Provincial Key Laboratory of Pharmaceutics;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education);School of Pharmacy, Guizhou Medical University; National Engineering Research Center of Miao’s Medicines, Guiyang 550004, China
  • Online:2017-10-27 Published:2017-10-27

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Abstract: To establish an ultra-performance liquid chromatography/quadrupole time-of-flight mass (UHPLC/Q-TOF MS/MS) method for analyzing the metabolites of main component in Inula cappa extract in rat bile after oral administration.Cannulas were surgically inserted into the bile duct of SD rats and bile was collected for 12 h after oral administration of active fractions of I.cappa at a dose of 100 g/kg of the material.After n-butyl alcohol precipitation treatment,the chemicals and their metabolites in rat bile were identified using UHPLC/Q-TOF MS/MS with selected reaction monitoring mode.The results revealed that twenty-seven metabolites were determined in bile,main metabolites are hydrogenation,methylation and glucuronidation of caffeoylquinic acid.It indicated that the chemicals can occur biotransformation in bile,the metabolic pathways mainly are methylation and demethylation glucuronidation.

Key words: Inula cappa, active fraction, UHPLC/Q-TOF MS/MS, metabolitescaffeoylquinic acid

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