Abstract: To study the anti-proliferative effect of mangiferin on mesangial cell with high glucose condition,and its role on mesangial cell autophagy.CCK8 method was used to detect the anti-proliferative effect of mangiferin on mesangial cell with high glucose condition.After treatment with 24 h,the expression levels of autophagy marker LC3-II protein in these five groups were detected by western blot.The GFP-LC3 green fluorescent trace method was used to detect autophagy of mesangial cells.The results showed that compared with high glucose group,Mangiferin treatment group (5×10^-5、1×10^-5、5×10^-6  mol/L) can significantly inhibit mesangial cell proliferation (P<0.01).Compared with high glucose group,LC3-II protein expression level of mangiferin treatment group (5×10^-5、1×10^-5 mol/L) was higher (P<0.05).Mangiferin treatment group (1×10^-5、2×10^-5、4×10^-5、8×10^-5 mol/L),the green fluorescent dot significantly increased.Mangiferin can inhibit the proliferation of mesangial cells with high glucose condition,its mechanism may be through improving the autophagy level of mesangial cell,maintaining the stability of environment in mesangial cell.

Key words: mangiferin, autophagy, mesangial cell, diabetic nephropathy