Abstract: With three kinds of common food-borne bacteria Staphylococcus aureus(ATCC25923),Escherichia coli(ATCC25922),Salmonella typhimurium(ATCC 14028)as testing strains,tube dilution quantitative method was used to detect the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of Eleutherine Americana (1 g/L).The antimicrobial diameters of eleutherine Americana extracts of different polarity solvents such as petroleum ether,ethyl acetate,chloroform,butyl alcohol,ethanol were also studied by drilling method.The results showed that the value of MIC and MBC from ethanol crude extract to Staphylococcus aureus were 3.91 mg/mL and 7.81 mg/mL.Different polar extracts of eleutherine americana had different degrees of antibacterial activity against these three bacteria,and were all highly sensitive to Staphylococcus aureus.The activity of petroleum ether extraction was the strongest,and the maximum antimicrobial diameter of the Staphylcoccus aureus was 31.83±0.50 mm.The results provided further reference for eleutherine americana extract in the development and application of natural food preservatives.

Key words: Eleutherine americana, different polarity extracts, food-borne bacteria, antibacterial effect