NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2019, Vol. 31 ›› Issue (6): 975-980.doi: 10.16333/j.1001-6880.2019.6.008

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Antibacterial effect and mechanism of dandelion phytic acid on Salmonella

GAO Fei-xiong,LIANG Yin-ku*,LI Yun-xiang   

  1. School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,723000,China
  • Online:2019-06-26 Published:2019-06-26

Abstract: To explore the antibacterial effect of dandelion phytic acid on Salmonella and its antibacterial mechanism.In this paper,the methods of precipitation and ion exchange were used to extract phytic acid from dandelion.The antibacterial effect of dandelion phytic acid on Salmonella was analyzed by filter paper method and the minimum inhibitory concentration of dandelion phytic acid was studied by dilution method.And the bacteriostatic mechanism of dandelion phytic acid against Salmonella was explored by analyzing the cell permeability and growth kinetics of Salmonella,combined with scanning electron microscopy and fluorescence microscopy.It indicated that dandelion phytic acid had good antibacterial ability against Salmonella and its minimum inhibitory concentration was 0.2 mg/mL.Moreover,the inhibitory effect of phytic acid on Salmonella was achieved by destroying cell membrane,and the higher the phytic acid concentration,the more significant the bacteriostatic effect.It is indicated that dandelion phytic acid can effectively inhibit the growth of Salmonella,mainly by destroying the cell membrane integrity of the cells,increasing the permeability of the cell membrane,and allowing the cell contents to overflow to inhibit the growth of bacteria.

Key words: dandelion, phytic acid, Salmonella, bacteriostatic characteristics, antibacterial mechanism

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